ABSTRACT
<p><b>OBJECTIVE</b>To sum up the clinical experience in the management of benign prostatic hyperplasia (BPH) with the prostate weighing over 80 ml by transurethral resection of the prostate (TURP) combined with 2 μm continuous-wave laser vaporesection (LVR).</p><p><b>METHODS</b>We retrospectively analyzed the clinical effects of TURP combined with 2 μm LVR in the treatment of 46 cases of BPH with the prostate volume > 80 ml.</p><p><b>RESULTS</b>All the operations were successfully accomplished. The operation time and intraoperative blood loss were (112.0 ± 20.0) min (range 86-176 min) and (77.9 ± 25.9) ml (range 50-200 ml), respectively. The catheters were withdrawn at 7 days after surgery. Transient urinary incontinence occurred in 6 cases and secondary hemorrhage was found in 2 postoperatively. Six-month follow-up revealed no urethral stricture or other complications. Compared with the baseline, the international prostate symptom score (IPSS) was significantly decreased at 6 months after operation (26.3 ± 1.8 vs 11.6 ± 1.7, P <0.05), and so were the quality of life (QOL) score (5.3 ± 0.7 vs 1.3 ± 1.1, P <0.05) and post-void residual urine (PVR) ([115.5 ± 55.6] ml vs [19.9 ± 11.6] ml, P <0.05). However, the maximum urinary flow rate (Qmax) was remarkably increased from (4.1 ± 2.6) ml/s to (16.2 ± 1.7) ml/s (P <0.05).</p><p><b>CONCLUSION</b>TURP combined with 2 μm LVR is safe and effective for the treatment of BPH with the prostate volume >80 ml.</p>
Subject(s)
Aged , Humans , Male , Middle Aged , Blood Loss, Surgical , Laser Therapy , Methods , Organ Size , Prostate , Pathology , Prostatic Hyperplasia , Pathology , General Surgery , Quality of Life , Retrospective Studies , Transurethral Resection of Prostate , Methods , Treatment Outcome , Urethral Stricture , Urinary Incontinence , Urinary RetentionABSTRACT
Objective: The chemical constituents in the methanol extract from the flowers of Trollius ledebouri were rapidly identified using UPLC-Q-TOF-MS in positive and negative ion modes. Methods: The analysis was performed on an ACQUITY UPLC BEH C18 reverse phase column (100 mm × 2.1 mm, 1.7 μm). The mobile phase consisted of methanol and 0.1% formic acid was used for gradient elution, 0-5 min, 95%-60% A; 5-13 min, 60%-45% A; 13-20 min, 45%-0% A; 20.1-25 min, 95% A. The flow rate was 0.3 mL/min, the injection volume was 2 μL. The information of the compounds was analyzed by the positive and negative ion mass spectra and elements composition. Results: The results indicated that 14 compounds in the extract from the flowers of T. ledebouri had been identified by direct comparison in both positive and negative ion mass data, they were vevatric acid, vanillic acid, orietin, orietin-2″-O-β-L-galactopyranoside, orietin-2″-O-β-D-xylopyranoside, quercetin, hyperoside, vitexin, apigenin, luteolin, acacetin-7- O-neohesperidoside, acacetin, pectolinarigenin, and salvigenin. Conclusion: UPLC-Q-TOF-MS could be used to analyze the constituents in the extract from flowers of T. ledebouri and to provide the protection for the quality control of the pieces as well as the stability and efficacy in the late stage clinical application, and to provide a reference for clarifying the material basis of its efficacy.
ABSTRACT
<p><b>OBJECTIVE</b>To study relatively pharmacological activities of cinnamon acid in blood serum of rabbit administered cinnamon acid, cinnamon and Jingui Shenqi pills.</p><p><b>METHOD</b>RP-HPLC determine and analysis blood serum sample from rabbits administered cinnamon acid, cinnamon and Jingui Shenqi pills. Condition of colour spectrum was Symmetry C18 (3.9 mm x 150 mm, 5 microm) chromato bar, mobile phase was methanol-1% glacial acetic acid water-solution (45:55), flow rate was 0.8 mL x min(-1), temperature of bar was 35 degrees C, detection wave length was 285 nm. The serum pharmacokinetic parameters were calculated with 3p87.</p><p><b>RESULT</b>Linear range of cinnamon acid is from 0.06-15 microg x mL(-1) (r = 0.9997), the lowest detectability is 0.054 microg x mL(-1). Pharmacokinetic process of cinnamon acid in rabbit could be all fitted to two-compartment model.</p><p><b>CONCLUSION</b>Sensitive and exclusive HPLC that adopt can exactly detect serum concentration in rabbits administerd cinnamon acid. Pharmacokinetic parameters of three conditions can reveal pharmacokinetics regularity of cinnamon acid in rabbit.</p>